Retatrutide Reference

GLP-1 / GIP / glucagon triple agonist · Phase 3 investigational

ClassGLP-1 / GIP / glucagon triple agonist
StatusPhase 3 investigational
Typical vials5 mg, 10 mg, 15 mg, 30 mg
Dose range2 mg to 12 mg
FrequencyOnce weekly
Half-life~6 days
Dosing unitmilligrams
RouteSubcutaneous injection

What Retatrutide is

Retatrutide is an investigational triple agonist (GLP-1, GIP, and glucagon receptors) from Eli Lilly, currently in Phase 3 trials and not FDA approved. Its Phase 2 results showed the largest average weight reduction reported for any incretin drug to date, which is why it dominates gray-market interest.

Because it is unapproved, there is no label to follow: the schedule below is the Phase 2 dose-escalation protocol, and everything else about real-world use (vial sizes, concentrations) is vendor convention, not a standard.

Reconstitution math

Concentration is everything: the same 10 mg vial gives a different draw for every amount of bacteriostatic water you add. The table uses the common 2 mL setup for each vial size, which puts every unit on a U-100 insulin syringe at 50 mcg for the default vial. Dashes mark draws too small to measure or larger than the syringe.

VialBAC waterConcentration2 mg4 mg6 mg8 mg12 mg
5 mg2 mL2.5 mg/mL80 u----
10 mg2 mL5 mg/mL40 u80 u---
15 mg2 mL7.5 mg/mL26.7 u53.3 u80 u--
30 mg2 mL15 mg/mL13.3 u26.7 u40 u53.3 u80 u
Retatrutide syringe drawsDRAW GUIDEEach dose as a filled syringe10 mg vial + 2 mL bacteriostatic water = 0.5 mg per 0.1 mL (10 units).0102030405060708090100units on a U-100 insulin syringe2 mg40 u (0.4 mL)4 mg80 u (0.8 mL)peprecon.com
Draw positions for the common doses at the default setup.

Dosing and protocol

Phase 2 TRIUMPH escalation: 2 to 12 mg weekly; some arms held at 8 mg.

The trials escalated every four weeks from 2 mg toward 8 or 12 mg weekly. Tolerability, not effect, sets the pace; some Phase 2 arms deliberately stopped at 8 mg. The half-life of about six days means steady state arrives around week four to five at any given step.

Retatrutide titration scheduleDOSINGThe published escalation, step by stepDose per injection (weekly), from the schedule cited below.0510mg159131721week246812maintenancepeprecon.com
Each step is held before escalating; the final dose continues as maintenance.

Pharmacokinetics

Retatrutide has published human pharmacokinetics: dose-proportional kinetics across the 0.5 to 12 mg range. The curves below are normalized one-compartment models built from the cited half-life, the same math the Planner uses. They show shape and timing, not absolute blood levels.

Retatrutide single-dose curvePHARMACOKINETICSOne dose, start to finishRelative level after one subcutaneous dose. Half-life ~6 days; about 97% cleared by 31 days.0%25%50%75%100%0102030days after injectionpeak ~4 dayshalf of peakpeprecon.com
Retatrutide accumulation with weekly dosingPHARMACOKINETICSThe same weekly dose builds for weeksRelative level with the same dose every week. Levels stop climbing around week 5.0%25%50%75%100%012345678weeks on the same weekly dosesteady statemarkers = weekly injectionspeprecon.com
Why week-one effects understate the eventual dose: levels build for weeks.

Storage and handling

Lyophilized vials keep best cold, dark, and dry; refrigeration is the community default and freezing lyophilized powder is common for long holds. After reconstitution, refrigerate at 2 to 8 C, do not freeze the solution, and date the vial. Bacteriostatic water's preservative keeps multi-dose use practical for about 28 days by USP convention, though our 120-day stability study found the preservative itself outlasts that window.

As an investigational compound there is no official storage guidance for vials. The community default mirrors the approved incretins: refrigerate after reconstitution, protect from light, and do not freeze the solution. Our lab-tested stability data for retatrutide is linked below when published.

Got a certificate of analysis with your vial? Run it through our COA Reader: it reads the report in plain English, checks purity and mass against the label claim, and links the testing lab’s own verification page. Our guide to reading a COA explains what the numbers do and do not tell you.

Sources

  1. Jastreboff et al., Triple-hormone-receptor agonist retatrutide for obesity, NEJM 2023
  2. Sanyal et al., Retatrutide for MASLD, Nature Medicine 2024

Related references: Tirzepatide · Semaglutide · Survodutide