Intranasal Peptide Dosing Calculator

Compute spray volumes for intranasal peptide preparations.

Mass in vial (mg)

Desired dose

Volume per spray (mL)

Sprays per dose

Vial mode tells you how many doses come out of a pre-reconstituted vial of known mass, and the total liquid volume needed for those sprays.

Powder mode tells you how much raw powder you'd need to weigh out for a target number of doses, and the total liquid volume your spray bottle should hold.

Spray volume helper

Most spray bottles need calibrating. Fill it with a known volume of water, count how many sprays it takes to empty, and this will tell you how much liquid comes out per spray.

mL of water

# of sprays

Volume per spray: — mL

Intranasal peptide dosing reference

How this calculator works

The liquid delivered per dose is the volume of one spray multiplied by the number of sprays per dose:

liquid per dose (mL) = volume per spray × sprays per dose

In vial mode, the calculator divides a reconstituted vial's mass by your dose to give the number of doses, then multiplies the dose count by the liquid per dose for the total solution volume. In powder mode, it multiplies your dose by a target number of doses to give the powder mass to weigh out, and reports the total volume your spray bottle should hold.

Calibrating spray volume

Most nasal pumps are not labelled with an exact actuation volume, so they need calibrating. Fill the bottle with a known volume of water, count the sprays it takes to empty, and divide:

volume per spray (mL) = water mL ÷ number of sprays

The spray-volume helper above performs this calculation.

Concentration per spray

Per-spray dose is the dose divided by the sprays per dose, and the solution concentration is the dose divided by the liquid per dose. Lowering the volume per spray or the sprays per dose raises the concentration needed to hit the same dose.

Nasal-specific handling

Intranasal absorption is more variable than injection, and the deliverable dose depends on the bottle priming correctly and the spray being retained in the nasal cavity. Doses are commonly split between nostrils.

Units & conversions

Doses are entered in micrograms or milligrams, where 1 mg = 1000 mcg. Volumes are in millilitres.

Storing intranasal solutions

Once reconstituted, most peptide solutions are refrigerated and used within a defined stability window; light and repeated temperature cycling degrade them. See PepRecon's bacteriostatic water stability data for the measured findings.

Data handling and privacy

Calculations run in your browser. Values are not transmitted or stored unless you explicitly generate a share link.

Scope & safety

PepRecon is a research and education resource. This calculator and reference material are provided for informational purposes and are not medical advice; they do not establish a dosing recommendation. Consult a qualified clinician before use.