SS-31 Reference

Mitochondrial-targeted tetrapeptide · Investigational (Phase 3 as elamipretide)

ClassMitochondrial-targeted tetrapeptide
StatusInvestigational (Phase 3 as elamipretide)
Typical vials10 mg, 50 mg
Dose range5 mg to 10 mg
FrequencyOnce daily
Half-life~3 hours
Dosing unitmilligrams
RouteSubcutaneous injection

What SS-31 is

SS-31 is the research name for elamipretide, a Szeto-Schiller peptide that concentrates in the inner mitochondrial membrane and binds cardiolipin, developed by Stealth BioTherapeutics through real Phase 2 and 3 trials in mitochondrial myopathy, Barth syndrome, and eye disease. It is a legitimate investigational drug with published human pharmacokinetics, which is rare for compounds in the longevity corner of the gray market.

Worth knowing: the clinical trials dosed 40 mg daily, several times the common community dose, so gray-market protocols are running well below the exposure the trial evidence describes.

Reconstitution math

Concentration is everything: the same 10 mg vial gives a different draw for every amount of bacteriostatic water you add. The table uses the common 2 mL setup for each vial size, which puts every unit on a U-100 insulin syringe at 50 mcg for the default vial. Dashes mark draws too small to measure or larger than the syringe.

VialBAC waterConcentration5 mg10 mg
10 mg2 mL5 mg/mL100 u-
50 mg2 mL25 mg/mL20 u40 u
SS-31 syringe drawsDRAW GUIDEEach dose as a filled syringe10 mg vial + 2 mL bacteriostatic water = 0.5 mg per 0.1 mL (10 units).0102030405060708090100units on a U-100 insulin syringe5 mg100 u (1 mL)peprecon.com
Draw positions for the common doses at the default setup.

Dosing and protocol

Clinical trials dosed 40 mg daily subcutaneously; community protocols run 5 to 10 mg daily.

Community protocols run 5 to 10 mg subcutaneously daily, usually in the morning. On a 10 mg vial with 2 mL of water, 5 mg is a 50-unit draw. With a half-life of a few hours there is no accumulation; daily dosing is about repeated exposure, not building a level.

Pharmacokinetics

SS-31 has published human pharmacokinetics: peak about one hour after a subcutaneous dose; half-life in the 2 to 4 hour range. The curves below are normalized one-compartment models built from the cited half-life, the same math the Planner uses. They show shape and timing, not absolute blood levels.

SS-31 single-dose curvePHARMACOKINETICSOne dose, start to finishRelative level after one subcutaneous dose. Half-life ~3 hours; about 97% cleared by 16 hours.0%25%50%75%100%051015hours after injectionpeak ~2 hourshalf of peakpeprecon.com

Storage and handling

Lyophilized vials keep best cold, dark, and dry; refrigeration is the community default and freezing lyophilized powder is common for long holds. After reconstitution, refrigerate at 2 to 8 C, do not freeze the solution, and date the vial. Bacteriostatic water's preservative keeps multi-dose use practical for about 28 days by USP convention, though our 120-day stability study found the preservative itself outlasts that window.

Got a certificate of analysis with your vial? Run it through our COA Reader: it reads the report in plain English, checks purity and mass against the label claim, and links the testing lab’s own verification page. Our guide to reading a COA explains what the numbers do and do not tell you.

Sources

  1. Karaa et al., Elamipretide in mitochondrial myopathy (MMPOWER-3), Neurology 2023
  2. Reid Thompson et al., Elamipretide in Barth syndrome (TAZPOWER), Genet Med 2021

Related references: MOTS-c · NAD+ · Thymosin Alpha-1