Glutathione Reference

Antioxidant tripeptide · Compounded injectable

ClassAntioxidant tripeptide
StatusCompounded injectable
Typical vials200 mg, 600 mg, 1,200 mg
Dose range200 mg to 600 mg
FrequencyA few times weekly
Half-lifeNo human data
Dosing unitmilligrams
RouteSubcutaneous injection

What Glutathione is

Glutathione is the body's principal intracellular antioxidant, a tripeptide of glutamate, cysteine, and glycine. Compounded injectable glutathione is common in antioxidant and skin protocols, typically a few hundred milligrams a few times per week subcutaneously or intramuscularly.

Reconstitution math

Concentration is everything: the same 600 mg vial gives a different draw for every amount of bacteriostatic water you add. The table uses the common 3 mL setup for each vial size, which puts every unit on a U-100 insulin syringe at 2 mg for the default vial. Dashes mark draws too small to measure or larger than the syringe.

VialBAC waterConcentration200 mg400 mg600 mg
200 mg3 mL66.6667 mg/mL---
600 mg3 mL200 mg/mL100 u--
1,200 mg3 mL400 mg/mL50 u100 u-
Glutathione syringe drawsDRAW GUIDEEach dose as a filled syringe600 mg vial + 3 mL bacteriostatic water = 20 mg per 0.1 mL (10 units).0102030405060708090100units on a U-100 insulin syringe200 mg100 u (1 mL)peprecon.com
Draw positions for the common doses at the default setup.

Dosing and protocol

Common antioxidant protocol: 200 to 600 mg, a few times weekly.

Doses of 200 to 600 mg are drawn in large volumes compared to peptides: at 200 mg/mL (a 600 mg vial in 3 mL), a 400 mg dose is a 2 mL injection. Oxidation is the practical enemy; reconstituted glutathione degrades faster than most compounds on this page, so small vials used quickly beat big vials stored long.

Pharmacokinetics

No curve is shown, on purpose.

Intravenous studies show glutathione clears plasma in minutes, and there is little rigorous data for the subcutaneous route. Plan by total weekly dose; a concentration curve has nothing honest to say here.

Storage and handling

Lyophilized vials keep best cold, dark, and dry; refrigeration is the community default and freezing lyophilized powder is common for long holds. After reconstitution, refrigerate at 2 to 8 C, do not freeze the solution, and date the vial. Bacteriostatic water's preservative keeps multi-dose use practical for about 28 days by USP convention, though our 120-day stability study found the preservative itself outlasts that window.

Got a certificate of analysis with your vial? Run it through our COA Reader: it reads the report in plain English, checks purity and mass against the label claim, and links the testing lab’s own verification page. Our guide to reading a COA explains what the numbers do and do not tell you.

Sources

  1. Richie et al., Randomized controlled trial of oral glutathione supplementation, Eur J Nutr 2015
  2. Allen and Bradley, Effects of oral glutathione on biomarkers of oxidative stress, J Altern Complement Med 2011

Related references: NAD+